Web3.5.1: Blotting. Blotting provides a means of identifying specific molecules out of a mixture. It employs three main steps. First, the mixture of molecules is separated by gel electrophoresis. The mixture could be DNA ( Southern Blot ), RNA ( Northern Blot ), or protein ( Western Blot) and the gel could be agarose (for DNA/RNA) or ... WebBlot stained with 0.1% Ponceau S in 5% acetic acid for 5 minutes according to the protocol (Panel B). Electrotransfer systems Several electrotransfer strategies exist. The most …
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