Chimeric repressor gene-silencing technology

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August undefined, 2024

WebSep 21, 2016 · In CRES-T, overexpression of a chimeric repressor, composed of the coding sequence of a transcription factor (TF) and short peptide designated as the repression domain, could interfere with the action of endogenous TF in plants. WebGuided by chimeric sgRNA, engineered Cas12b facilitates robust genome editing in human cells and mice ... (NMD), thus silencing gene expression (130, 131). Gene expression can also be shut down by ABE-mediated destruction ... Advances in CRISPR/Cas gene-editing technology have led to new therapeutic options for a wide range of genetic and ...

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WebOct 21, 2016 · Chimeric repressor gene silencing technology (CRES‐T) is a novel gene silencing system in which a dominant negative repressor of a TF suppresses the target genes of endogenous TF and its functionally redundant TFs (Hiratsu et al., 2003). WebA chemiresistor is a material that changes its electrical resistance in response to changes in the nearby chemical environment. Chemiresistors are a class of chemical sensors that … phil in the blanks website

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WebApr 12, 2024 · Base editing, first reported in 2016, is capable of installing C•G to T•A and A•T to G•C point mutations, while largely circumventing some of the pitfalls of traditional CRISPR/Cas9 gene editing. Despite their youth, these technologies have been widely used by both academic labs and therapeutics-based companies. WebJan 1, 2011 · Chimeric REpressor gene Silencing Technology (CRES-T) is a useful tool for functional analysis of plant transcription factors. In this system, a chimeric repressor … WebDefective silencing of retrotransposable elements has been linked to inflammageing, cancer and autoimmune diseases. However, the … phil in the blanks youtube

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WebIn this system, a chimeric repressor that is produced by fusion of a transcription factor to the plant-specific EAR-motif repression domain (SRDX) suppresses target genes of a transcription factor dominantly over the activity of endogenous and functionally redundant transcription factors. WebChimeric REpressor gene-Silencing Technology (CRES-T) is a powerful gene-silencing tool to analyze the function of Arabidopsis transcription factors. philinthegaps.as.meWebDec 5, 2013 · We produced transgenic Arabidopsis plants that express chimeric genes for transcription factors converted to dominant repressors, using Chimeric REpressor gene-Silencing Technology (CRES-T), and evaluated the salt tolerance of each line. The seeds of the CRES-T lines for ADA2b, Msantd, DDF1, DREB26, AtGeBP, and ATHB23 … philinthegaps

"WebMar 10, 2014 · Arabidopsisplants transformed with a chimeric repressor for 6 transcription factors (TFs), including ADA2b, Msantd, DDF1, DREB26, AtGeBP, and ATHB23, that … " - Chimeric repressor gene-silencing technology

Chimeric repressor gene-silencing technology

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WebSep 28, 2024 · Genome editing technology has become one of the hottest research areas in recent years. Among diverse genome editing tools, the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins system (CRISPR/Cas system) has exhibited the obvious advantages of specificity, simplicity, and flexibility over any … WebMasaru Ohme-Takagi. Chimeric repressor gene-silencing technology (CRES-T) is a powerful tool that has recently been developed for the functional analysis of plant transcription factors and for the ...

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WebDec 1, 2024 · The constructs used for overexpression and Chimeric REpressor genes Silencing Technology (CRES-T) for each gene were based on modified vectors derived from pGreenII0029 (Hellens ... We surveyed almost 20 000 transgenic lines that expressed the chimeric repressor of 1700 independent Arabidopsis transcription factors and …

WebAug 3, 2024 · For explaining the molecular mechanism of two-layered petaloid tepal development, the modified ABC model has been proposed, in which B class genes … WebApr 11, 2024 · Yeasts have been a part of human life since ancient times in the fermentation of many natural products used for food. In addition, in the 20th century, they became powerful tools to elucidate the functions of eukaryotic cells as soon as the techniques of molecular biology developed. Our molecular understandings of metabolism, cellular …

WebMar 28, 2016 · The chimeric repressor gene silencing technology (CRES-T) system converts a transcriptional activator into a strong repressor by fusion to the SRDX repression domain; this process induces a phenotype similar to loss-of-function alleles ( 27) and can also confer tolerance to various abiotic stresses ( 28 ). WebAug 11, 2013 · In poplar, SND2 subfamily (NAC-q) contains nine putative counterparts (Hu et al. 2010), which may interfere with the efforts to identity their functions by gene-knockout or antisense technology. Chimeric repressor silencing technology has been used as a powerful tool to analyze the functions of redundant plant transcription factors (Hiratsu et ...

WebDec 5, 2013 · We produced transgenic Arabidopsis plants that express chimeric genes for transcription factors converted to dominant repressors, using Chimeric REpressor gene …

WebCIN-type TCP genes have redundant Chimeric repressor gene-silencing technology (CRES- molecular functions to regulate margin development T) has provided unique transgenic ornamental flowers (Koyama et al. 2007). A chimeric repressor, (Nakatsuka et al. 2011; Shikata et al. 2011; Tanaka et al. TCP3SRDX, which consists of Arabidopsis … phil in the futureWebSep 12, 2013 · Chimeric repressor gene-silencing technology (CRES-T) provides a powerful tool for dominant negative genetic modification of a transcription factor. In CRES-T, a short but strong, ... phil in the gapsWebMar 3, 2024 · National Center for Biotechnology Information philinthe twitterWebChimeric REpressor gene-Silencing Technology (CRES-T) was developed as a specific technology for gene silencing, mainly used for analyzing the function of plant … philintl.comWebChimeric REpressor gene-Silencing Technology (CRES-T) is a powerful gene-silencing tool to analyze the function of Arabidopsis transcription factors. phil in the gaps social workWebMay 29, 2024 · Repressor element 1 silencing transcription factor (REST) is a neuronal repressor gene that regulates neuronal stem cell differentiation [11,12]. REST also plays a multifunctional role in the regulation of non-neurogenic cells [ 13 , 14 ]. phil in the gaps llcWebAug 3, 2024 · Recently, we have obtained results directly supporting the modified ABC model in the liliaceous plant Tricyrtis sp. by chimeric repressor gene-silencing technology … phil in the blanks worksheets