Cst atf4 antibody
WebATF4 Rabbit pAb (A0201) Western blot analysis of extracts of HeLa cells, using ATF4 antibody (A0201) at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours. Secondary antibody: HRP Goat Anti … WebDec 17, 2024 · BackgroundThe role of activating transcription factor 4 (ATF4) underlying gastric cancer (GC) remains unclear. The purpose of this study was to investigate the expression levels and biological functions of ATF4 in GC.MethodsExpression of ATF4 was detected by quantitative PCR (qPCR), Western blotting, and immunohistochemistry. Cox …
Cst atf4 antibody
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WebPurified anti-ATF4 Antibody. Total cell lysates (15 µg protein) from non-treated and Tunicamycin (Tm) treated (2 µg/mL, six hours) HeLa and 293T were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-ATF4 (clone W16016A) antibody or competitor's antibody used at the ... WebMar 3, 2024 · In the nervous system, activating transcription factor 4 (ATF4) is involved in the regulation of synaptic plasticity and memory formation. Here, we show that ATF4 …
WebSep 5, 2024 · ATF4 is induced in CTLs exposed to tumor-derived factors and antigen stimulation. We sought to determine the expression of ATF4 in CD8 + T cells upon their activation or exposure to the stress stimuli and tumor-derived factors. Treatment of mouse splenic CD8 + T cells with thapsigargin, an inducer of the endoplasmic reticulum stress … WebThis antibody has been shown to work in applications such as: Precipitation, Sequencing, ChIP-Seq, ChIP, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot, and Other. ATF-4 (D4B8) Rabbit …
WebJun 24, 2024 · PERK IPs were performed with 1 mg of lysate incubated with 2 µg of primary antibody (Proteintech, #20582-1AP) or the corresponding IgG (Cell Signaling Technology, #2729) overnight at 4 °C with ... WebOverview Product name Anti-ATF-4 antibody See all ATF-4 primary antibodies Description Rabbit polyclonal to ATF-4 Host species Rabbit …
WebOct 20, 2024 · ChIP assays with NFATc1 or ATF4 antibody demonstrated that isoproterenol increased NFATc1 and ATF4 binding to their cognate binding elements in RANKL ... MA, USA). CREB1 and phospho-CREB1 antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA), and phospho-ATF4 antibody was …
WebOct 22, 2010 · ATF4 is a transcriptional activator of the integrated stress response, a program of gene expression involved in metabolism, nutrient uptake, anti-oxidation, and … tsuredure children gogoanimephn application brnWebHowever, CS-11815 detects a band with a mass of ~49 kDa, while the expected molecular weight of ATF4 is ~38 kDa. In contrast, SC-200 is a very dirty polyclonal antibody, but it seems to detect ... tsuredure translateWebImmunoprecipitation of ATF-4 from extracts of 293 cells, treated with tunicamycin (2 μg/ml, 8 hr), using Rabbit (DA1E) mAb IgG XP ® Isotype … tsuredure children malWebOct 22, 2010 · ATF4 is a transcriptional activator of the integrated stress response, a program of gene expression involved in metabolism, nutrient uptake, anti-oxidation, and the activation of additional transcription factors, such as CHOP/GADD153, that can induce apoptosis. ... (Cell Signaling Technologies). ATF4 antibody was prepared against … tsuredure children english castWebMar 25, 2024 · Some oncoproteins along with stress kinase general control non-derepressible 2 (GCN2) can ensure the induction of activating transcription factor 4 (ATF4) to counteract amino acid deprivation; however, little is known regarding the role of the oncogenic EGFR-PI3K pathway. In this study, we demonstrate that both mutated EGFR … phn application 2022 pacWebSep 29, 2024 · ATF-4 Antibody (B-3) is an IgG 2b κ mouse monoclonal ATF-4 antibody (also designated ATF4 antibody, cAMP-dependent transcription factor ATF-4 antibody, CREB-2 antibody or cAMP … phn assurance framework